Comparison of autofocus methods for automated microscopy
نویسندگان
چکیده
منابع مشابه
Digital autofocus methods for automated microscopy.
Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.
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Marı́a Cristı́na Chávez-Sánchez Centro de Investigación en Alimentación y Desarrollo, A. C. Unidad Mazatlán en Acuicultura y Manejo Ambiental Sábalo Cerritos S/N Apdo. Postal 711 Estero del Yugo Mazatlán Sinaloa, C.P. 82010, México Abstract. We present a new algorithm to determine, quickly and accurately, the best-in-focus image of biological particles. The algorithm is based on a one-dimensional...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1991
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990120302